Djed Spence told the England camp he is dealing with a muscular niggle as the team prepares for the last‑16 tie with Mexico at the Azteca Stadium.
The 25‑year‑old started the round‑of‑32 win over DR Congo at right‑back and featured in England's group‑stage games, but his fitness remains uncertain for the Sunday night match (Monday 01:00 BST).
England also have Reece James doubtful after a hamstring injury, while centre‑back Jarell Quansah has been cleared to play after recovering from an ankle issue in the group‑stage win over Panama.
Thomas Tuchel will have to decide whether to keep Spence, shift to a left‑back role or rely on alternatives such as Ezri Konsa if the defender cannot start.
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